Population Genetics of the Lyme Disease vector, Ixodes scapularis

The tick species Ixodes scapularis (Acari: Ixodidae) is the main vector in the United States for Borrelia burgdorferi, the causative agent of Lyme Disease. Previous research has identified behavioral differences between northern and southern populations of I. scapularis with northern nymphs spending more time above leaf litter increasing the likelihood for human contact. This difference in behavior is observed despite the environment, suggesting an unknown genetic driver to these behavioral patterns. This study will expand on a pilot study using ticks from Connecticut, South Carolina, and Minnesota that identified 99,187 SNPs distributed across 14,168 polymorphic loci using triple-enzyme restriction-site-associated DNA sequences (3RAD). This illustrates that I. scapularis populations have large amounts of intra- and inter-population variation. In this upcoming study we plan to assess 27 populations across the range of I. scapularis to elucidate the genotypes driving behavioral differences and Borrelia transmission. We are also interested in microbiome disparities across this range as it could have an impact onBorrelia transmission. The student will be trained and involved across the pipeline of this study: tick ID, DNA extraction, 3RAD and 16S library prep, and bioinformatics analysis.

Project mentors: Julia Frederick and Travis Glenn
Type of project: Combination of Empirical (lab-based) and Quantitative (computer-based)