Understanding hydrogen peroxide employment by honeybees as an anti-parasitic social immunity tool

Hydrogen peroxide has immunological function across a broad suite of life on earth, and is still used in the US as a common antiseptic. Honeybees are another self-medicating social animal also known to use hydrogen peroxide manipulation – mostly in honey production; this in part makes it the only naturally occurring foodstuff to never spoil and underpins part of its medical applications in wound healing. Previous undergraduate work with UGA and Emory University investigated whether hydrogen peroxide content in honey poses a toxicity risk to a macroparasite, the small hive beetle, in addition to its antimicrobial effects. Dr Lewis Bartlett is currently trialling novel small hive beetle control methods, and this project will integrate with that work to further establish: ranges of hydrogen peroxide content in naturally occurring honey across different nectar sources, honeybee tolerance of hydrogen peroxide consumption and whether it exceeds that of other insects, and toxicity of hydrogen peroxide to juvenile and adult small hive beetles with the potential for it to be employed as a parasite control agent. The study will further be framed in the context of floral diversity and pollinator health, by determining whether different plants favour the production of honey with different antiparasitic or antiseptic properties through. The student will undertake empirical entomological toxicity trials and basic entomological rearing at the UGA honey bee lab, as well as data analysis and visualisation focussed on assessing survivorship, with the aim to produce a concise scientific publication; they will also have the opportunity to learn field skills in apicultural research if they wish. The student will also be given the option to attend (for free – including transport from Athens, meals and accommodation) a leading honeybee conference in North Georgia (https://bees.caes.uga.edu/yhc-uga-beekeeping-institute.html) as an introduction to the system in the week of May 11th – 16th, prior to the official REU start date of May 18th.

Mentor: Lewis Bartlett
Project Type: Empirical, lab- and field-based

Specific Requirements: Student cannot have a known allergy to honeybees, and must have no objection to euthanasia or experimental destruction of live insects for research purposes.

Honeybees in a hive